Team:Newcastle/Meetings/26 March 2010
From 2010.igem.org
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Formal meeting - 26th March 2010
- Room: CBCB; Start: 11 am
- Chair: Jannetta, Minutes: Rachel, Computer: Harsh
- Present: Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh
- Apologies: Phil (Holiday), Jem and Matt
- Approval of agenda and minutes - Approved
3. Brief summary of previous meeting |- |Tutorial |-
| 4. Research Feedback
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|1. Properties of concrete and surface tension (Zoltan and Steven)
High compressive strength low tensile strength Steal added good for temp change Caco3 – similar to concrete; expand same as concrete... might crack like concrete Spider silk like steal- reduce possibility of cracking Glue- no many papers- expansion may not be a problem? Silk alkali resistant Capillary properties of concrete change over time- in project we consider small rapid Cement water ratio and surface area and environment Where to look for the cracks? Crack pattern |-
| 2. Microinjection (Zoltan and Steven)
|- |Simple issue Not correct term Swimming Bacillus Oxygen gradient big selling points |-
|3. Genetics Homologous recombination plasmid chromosome (Harsh and Rachel)
|- |Test in plasmids- Find good plasmid Integrate into genome Recombination site... Find!!! amyE site amylase with insert can’t break down amylase |-
|4. Nutrients (Harsh and Rachel)
|- |Bacteria algae growth living in concrete Only activate feed bacillus Two hybrid Bio films exclude other not algae Antifungals, anti algals, Antibiotic |-
|5. Sporulation, germination and secretion control (Alan and Da)
|- |SpoA – last years team sporulation control, maybe turn off completely Nutrient availability KinA Inducible promoter |- |Glue out of bug? |-
|6. Quorum sensing (Younus and Phil)
|- |Compx And cff Two component system comp |-
|-7. Kill switch (Richard and Jannetta)
|- |Why ? Don’t want?- not die after job fill new cracks Nuclease Phil masEF Skf SbB If linear DNA works |-
|8. Glue, CaCo3 and spider silk (Zoltan and Jannetta)
Bacteria that produce CaCo3 from urea How secrete spider silk long complex proteins – how can we make it happen in bacillus Secretion systems |-
|9. Other points
|- |Linear DNA – Richard Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though |-
|5. Action points
|- |All -Read Voygt paper |- |All-Next week working off the proper wiki!!! |- |Janetta contact details doc in drop box |- |Janetta and Richard – Flow chart!!! |- |Reminder to neil and jen not welcome trust funding by 2nd of April? |- |Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design |- |Janetta- send us links for graphic design software- please |- |Transfer the wiki |-
|1. Individual iGEM applications
|- |Phil hasn’t applied |- |Richard not accepted |- |Janetta text Phil |- |Jen/Neil-Pay for team!! |-
|2. Visas/Passport update
|- |Harsh- May June apply |- |Richard visa waiver |-
|3. Wiki transfer
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|1. Clarify contents of identified pages
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|2. Id Ethics page
|- |Research page |- |Safety page |- |entify other pages required |-
|3. Ideas for layout / theme
|- |Zoltan Tidy it up-Urgent |- |Borrow last years / Look at anything thats nice |- |It evolves!!! We can change it!!! Now!!!!!!! |-
|4. Travel plans
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|1. Flights
|- |Need to sort out who is going |-
|2. Accomodation
|- |Need to sort out who is going |-
|5. Funding
|- |Harsh has made draft letter |- |Main chemical company – free stuff ?
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|6. Advisors/additional team members - civil engineer/graphics designer?
|- |Still looking –Neil,Jen |-
|7. Tutorials
|- |Sequentia and Clone manager- basics |- |Modelling part two |-
|8. What next?
|- |Flow chart of what we’re going to do Big picture!!! |- |If its coloured life is easier |- |Cambridge brick-Colour |- |Web services for Biosynthetics, Flow chart |- |Deverner |-
|9. Time line discussion
|- |Need someone else on time line. |- |16th of June Lab induction |- |Deadlines soon |- |Change flight deadline – not 31st
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|6. Other business
|- |Pdf mark up tools – pdf exchange viewer, foxit, keeps mark up |- |Making dropbox use better |-
|7. Items for next agenda
|- |Need wiki transferred by next meeting!!! |- |Review of flow chart if done |-
|8. Next meeting- Thursday early as possible – email organise 1. Chair-Da 2. Minutes-Alan 3. Computer-Younus|-