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TEAM ALBERTA
HOME
AT A GLANCE
PROJECT
Overview
BioBytes 2.0
Software
Modelling
The Kit
Kit Components
ACHIEVEMENTS
Overview
Medal Requirements
Media
HUMAN PRACTICES
Overview
Distribution Analysis
High School
DiscoverElle Visit
Safety
PARTS
TIMELINE & PROTOCOLS
Overview
General Protocols
Reusable Plates
Base Plasmids
Creating Parts
Transforming Cells
Anchor
Beads
Optimizations
Software
TEAM
List of General Protocols
Number
Title
0
Metaprotocol for adding and modifying University of Alberta iGEM protocols
1
General Advice
2
Transformations
3
5 mL Overnight
4
Glycerol Stock
5
Plasmid Miniprep
6
Restriction Digest
7
Agarose Gel Electrophoresis
8
Gel Extraction
9
Ligation
10
Colony PCR
11
PCR Purification
12
Sample labelling conventions
13
Vector dephosphorylation protocol
14
Fluorescent sequencing reaction
15
LB plates and broth
16
Primer design
17
PCR
18
In vitro BioByte assembly