From 2010.igem.org
Protocols
In this section we will offer protocols for techniques we have used that are not part of comerically avalible kits.
Ligase Free Cloning
This technique uses e. coli ligase to ligate a gene of interest with a plasmid backbone that both have compatible sticky ends.
Step 1
Information
Step 2
Information.
Protocol 2
about
Step 1
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Step 2
Information.
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