Team:Newcastle/Meetings/12 July 2010
From 2010.igem.org
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Informal meeting - 12th July 2010
- Chair: Da, Minutes: Younus
- Walton Library [3.30 - 5.00pm]
Cloning Strategies
5 parts to a Cloning Strategy:
The cloning strategies for each biobrick should contain the following sections in this order:
- Purpose and Justification
- Modelling
- Construction
- Cloning and Integration
- Testing and Characterising
Required Cloning Strategies
There are 8 cloning strategies which are required:
- SR1 - finished!
- YneA - biobrick nearly coming back - our most important biobrick!
- LacI - not sure how to test this biobrick
- 2 for Swarming (sfp and swrA) - cloning strategies nearly finished
- 2 for Subtilin (immunity and production) - cloning strategies nearly finished
- RocF (need to use Gibson method) - not started
So only the cloning strategy for SR1 is finished. 7 more to go!
Allocation of team members to cloning strategies:
- YneA - Phil, Rach, Deena, Younus
- LacI - Phil, Younus, Rachel
- Swarming (2) - Harsh, Alan, Steve, Da
- Subtilin (2) - Harsh, Alan, Steve, Deena
- RocF - Harsh, Alan, Steve, Da
Wiki
Labbook
Everyone needs to carry out the following protocol for each day in the lab
- Aim
- Materials and Methods
- Procedure/Protocol
- Results
- Conclusion
Relevant photographs should be added and labelled
General:
- Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)
UK iGEM Gathering at Newcastle - 20th/21st July
Presentation:
Introduction
Rach, Deena, Jannetta [2 mins]
- Cover Page
- Picture of us standing up
- Introduction of our iGEM project - using BS to repair concrete
General Outlook
Harsh, Da, Phil [5 mins]
- Starting with bioreactor
- Transformation into spores
- Transportation
- Spraying on building
- Germination
- sensing crack + non-environmental kill switch
- swarming
- end of crack signalling = QS
- Filamentous cells
- CaCO3
BioBrick Discussion
Alan, Younus, Steve [8 mins altogether; 2 mins each]
- Swarming
- Subtillin
- Filamentous cells - YneA
- CaCO3 - Urease
- LacI
Whats next...
[5 mins remaining] 'Save the babies!!!'
Minideadlines
- Presentation slides to be e-mailed by tomorrow (Wed) 5 pm
- Cloning Strategies - to be completed by Friday, 16th July 2010.
- YneA
- One sequence for sfp primers and coding sequence ready (Goksel)
- Subtilin just immunity
Action Points
- Finish wiki updates by Sunday 18th (everyone)
- Team page on Wiki:
- Add Colin Harwood, Colin Davies, Wendy Smith and Goksel Misrili to list of advisors
- Deena, Phil and Younus to write about yourself + pictures