Team:Harvard/flavor/parts

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parts and primers

Primers

NameDescription
J45004_FWintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer
J45004_RWintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer
J45017_FWintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer
J45017_RWintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer
NOSterm_BB_FPlant specific terminator forward primer
NOSterm_BB_RPlant specific terminator reverse primer
Stop_NOSterm_BB_FNOSt plant specific terminator with stop codon on end
pENTCUP2_BB_FPlant specific promoter forward primer
pENTCUP2_BB_RPlant specific promoter reverse primer

Parts

NameDescription
pENTCUP2 promoterPlant specific promoter. Used to drive expression of our flavor constructs in plants.
NOSt terminatorPlant specific terminator. Terminates transcription but no stop codon on end.
NOSt terminator + stopPlant specific terminator. Terminates transcription with stop codon on end.
BrazzeinSweet-tasting protein. 54 amino acid sequence.
MiraculinTaste-inverting protein turns sour to sweet. 191 amino acid sequence
Brazzein YFP2x C-terminusBrazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression
Miraculin YFP2x C-terminusMiraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression
Brazzein StrepII C-terminusBrazzein with StrepII tag on C-terminus for Western Blot.
Miraculin StrepII C-terminusMiraculin with StrepII tag on C-terminus for Western Blot.

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