Team:Brown/Protocols/Restriction Digest

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Restriction Digest protocol

Brown iGEM 2010, modified from Knight Lab protocol.

Materials

  • Restriction enzymes (we use NEB)
  • NEB buffer 2/3 (use buffer finder on NEB website. Buffer 2 is standard for iGEM enzymes)
  • BSA
  • Deionized, sterile H2O

Digest mix

The reaction below is a 50ul reaction, 100ul reactions are also common if you want your DNA to be dilute.

  • 5ul NEB Buffer 2
  • _ul DNA (usually ~500ng)
  • 0.5ul 100x BSA
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