Team:Imperial College London/Chassis

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Chassis

Our choice of chassis was B. subtilis.

Icbsub.jpg
Bacillus | Breakdown
IC 2010 IGEMchassis.jpg

E.coli was considered as a possible option. Despite the gram negative outer membrane, there are strains that have been made more permeable through knockout of Lipid A biosynthesis in lipopolysaccharides [4] There are also proteins that can disrupt membranes when they are inserted in them, thus making them more permeable. These chages in permeability are likely due to transient ruptures of outer membrane and so unlikely to make a very responsive or robust detecton organism [4].

References

Surplus information on B. Subtilis chassis [http://www.subtiwiki.uni-goettingen.de/wiki/index.php/Main_Page Subti-Wiki] 

[http://mic.sgmjournals.org/cgi/content/full/146/12/3025 1]
[http://www.microbialcellfactories.com/content/5/1/22 2]
[http://www3.interscience.wiley.com/cgi-bin/fulltext/118603263/PDFSTART 3]
[http://aac.asm.org/cgi/content/full/43/6/1459?view=long&pmid=10348770 4]
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