Project/Venus/

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Contents

Characterisation of the fluorescent reporter protein - Venus - [http://partsregistry.org/Part:BBa_K354002 BBa_K354002]

BioBrick Background


Sequence Development and Annotation

As was the case in the assembly of the PlcR-PapR BioBrick, the synthesized Venus BioBrick was received in the pMK plasmid (below). PMK-Venus.JPG

Once digested with EcoR1 and Pst1, the Venus fragment was recovered via gel extraction and purified in preparation for its ligation into PSB1C3. Lane 2 houses the Venus digest with the excised fragment shown (782bp).
Venus digest.JPG


Testing and verification