Team:St Andrews/project/laboratory/protocols

Contents 1 Protocols 1.1 Cell Culture 1.2 Transformation 1.3 Restriction Digest 1.4 Ligation 1.5 PCR 1.6 DNA Extraction 1.7 Annealing of Complementary Primers

Protocols

We used a number of protocols from OpenWetWare in addition to the manufacturer's recommended protocols for our materials.

Cell Culture

OpenWetWare [http://openwetware.org/wiki/Bacterial_cell_culture Bacterial Cell Culture]

Transformation

NEB Turbo Competent E. coli

Clontech Stellar Competent E. coli.

Restriction Digest

Promega Enzymes

NEB Biobrick Assembly Kit (Gingko Bioworks)

Ligation

NEB Biobrick Assembly Kit (Gingko Bioworks)

PCR

OpenWetWare [http://openwetware.org/wiki/Knight:Colony_PCR Knight - Colony PCR]

NEB Taq

Finnzymes Phusion

DNA Extraction

Quiagen Miniprep Isopropanol Extraction of small DNA fragments

Annealing of Complementary Primers

OpenWetWare [http://openwetware.org/wiki/Endy:Annealing_complementary_primers Endy - Annealing Complementary Primers]