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Team:UCL London/Our BioBricks
From 2010.igem.org
NEW PARTS CHARACTERISED
The following table is that of all the Biobricks and parts that we have used over the course of iGem 2010. This does not necessary mean that they were all incorporated into the final product!
| Part Code | Part Name | Location | Backbone | Length(bp) |
|---|---|---|---|---|
| R0010 | PROMOTER (lacI Regulated) | Plate1_1D | pSB1A2 | 200 |
| R0010 | PROMOTER (lacI Regulated) | Plate1_1D | pSB1A2 | 200 |
EXISTING PARTS/DEVICES CHARACTERISED
| Part Code | Part Name | Location | Backbone | Length(bp) |
|---|---|---|---|---|
| BBa_K239011 | Stress Light GFP Anaerobic Metabolism Detector 2 | 972 | ||
| K239009 | GFP test devise for Spy promoter | 1045 | ||
| K239015 | GFP test devise for DegP promoter | 1116 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K239011|UCL_London 2009 iGEM BBa_K239011 PART] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K239009|UCL_London 2009 iGEM BBa_K239009 PART] [http://partsregistry.org/wiki/index.php?title=Part:BBa_K239015|UCL_London 2009 iGEM BBa_K239015 PART]
Parts Submitted
| Part Name | Part Type | Description | Designer | Length(bp) |
|---|---|---|---|---|
| BBa_K301001 | Regulatory | Tac promoter, hybrid derived from trp and lac promoters | Xiang Chen | 73 |
| BBa_K301001 | Device | pTAC with RFP reporter | Xiang Chen | 942 |
Check out our parts page to see the parts submitted in more detail [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=UCL_London&Done=1|UCL_London 2010 iGEM Team Parts].
OTHER PARTS/DEVICES USED
| Part Code | Part Name | Location | Backbone | Length(bp) |
|---|---|---|---|---|
| R0010 | PROMOTER (lacI Regulated) | Plate1_1D | pSB1A2 | 200 |
| K239005 | Nark | Eurogentec | Amp | 139 |
| K239006 | mNark | Eurogentec | Amp | 89 |
| R0079 | Promoter (LasR & PAI regulated) | Plate1_19P | pSB1A2 | 936 |
| K274100 | VRDP | Plate3_6H | pSB1A2 | 3385 |
| B0015 | Double Terminator | Plate1_23L | pSB1AK3 | 129 |
| pSB1K3 | Kanamycin Resistance | Plate1_5A | pSB1K3+J04450 insert | 2206 |
| pSB1T3 | Tetracycline Resistance | Plate 1_7A | pSB1T3+J04450 insert | 2463 |
| pSB1C3 | Chloramphenicol Resistance | Plate 1_3A | pSB1C3+J04450 insert | 2072 |
| R0011 | Promoter (lacI regulated, lambda pL hybrid) | Plate1_6G | pSB1A2 | 55 |
| B0034 | RBS | Plate1_2M | pSB1A2 | 12 |
| C0079 | lasR activator from P. aeruginosa PAO1(+LVA) | Plate1_14J | pSB2K3 | 756 |
| pSB1AK3 | Amp+Kana | Plate1_11A | +J04450 insert | 3189 |
| pSB1AT3 | Amp+Tetra | Plate1_13A | +J04450 insert | 3446 |
| pSB1AC3 | Amp+Chlora | Plate1_9A | +J04450 insert | 3055 |
| K274200 | CrtEBIY with rbs (Orange) | Plate3_6L | pSB1A2 | 4555 |
| K274002 | Purple | Plate3_12B | pSB1T3 | 7345 |
| K274003 | Dark Green | Plate3_20H | pSB1K3 | 6032 |
| row 15, cell 1 | row 15, cell 2 | Plate3_20D | pSB1T3 | 6032 |
| K274004 | Light Green | Plate3_20J | pSB1K3 | 6200 |
| Plate3_22F | pSBT3 | 6200 | ||
| I712069 | RBS+T7 RNAP | Plate2_13K | pSB1AK3 | |
| I719005 | T7 promoter | Plate1_15N | pSB1A2 | |
| I712074 | T7 strong pro | Plate1_6N | pSB1AK8 | |
| I13507 | RBS+RFP+T | Pate1_22O | pSB1A2 | 861 |
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