Korea U Seoul/2 October 2010

From 2010.igem.org

Revision as of 13:45, 20 October 2010 by Needks (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Ligation of each parts : PyodA-mAAA-PzntA-RFP, ParsR-GFP and pSB1C3


Reagent Volume (μL)
10x T4 DNA ligase reaction buffer 2
T4 DNA ligase 2
Each of the digests 2 + 2 + 2 = 8
D.W. 8 / total = 20
Incubation at room temperature for 30min


and stop at 80˚C for 20min

Retrieved from "http://2010.igem.org/Korea_U_Seoul/2_October_2010"