Promoters

The DREB1C iGEM promoter is derived from a transcription factor that is up-regulated by cold stress and down-regulated by circadian controls to prevent plant growth retardation due to the buildup of <a href="https://2010.igem.org/Team:Nevada/DREB1CPromoter">DREB1C</a> (Dehydration Response Element Binding Protein) during the day (the cause of dwarfism). The promoter region for <a href="https://2010.igem.org/Team:Nevada/DREB1CPromoter">DREB1C</a> begins just upstream of the translational start sequence (ATG) and contains 441bp of the upstream sequence with six cis-acting, ADA independent elements for the up-regulation during cold stress and circadian down-regulation.

Kazuo Nakashima and Kazuko Yamaguchi-Shinozakia, Regulons involved in osmotic stress-responsive and cold stress-responsive gene expression in plants, Physiologia Plantarum 126: 62–71. 2006

Satoshi Kidokoro, Kyonoshin Maruyama, Kazuo Nakashima, Yoshiyuki Imura2, Yoshihiro Narusaka3, Zabta K. Shinwari4, Yuriko Osakabe, Yasunari Fujita, Junya Mizoi, Kazuo Shinozaki, and Kazuko Yamaguchi-Shinozaki, The Phytochrome-Interacting Factor PIF7 Negatively Regulates DREB1 Expression under Circadian Control in Arabidopsis, Plant Physiol. Vol. 151, 2009

We would like to thank the following sponsors for their support in helping us make this project possible. Much thanks to the [http://www.cabnr.unr.edu/Students/Majors.aspx Departments of Biochemistry and Biotechnology] and the [http://www.cabnr.unr.edu/Students/Glance.aspx College of Agriculture, Biotechnology and Natural Resources] for their encouragement and support. Thank you [http://www.unr.edu/inbre/ Nevada INBRE] for over $6,000 in support for supplies and registration costs. Thank you to Associated Students of the Univeristy of Nevada for supporting our fund raising efforts. Thank you to [http://www.promega.com/Catalog/CountrySelect.aspx?returnurl=/Default.asp Promega Co.] for free enzyme donations. Thank you to [http://www.invitrogen.com/site/us/en/home.html?cid=covinvggl89100000002336s& Invitrogen Co.] for a discount on our Vector NTI program.