Lab notes (10/11 - 10/17)

Flagella Group

Transformation of FlhDCm composite part in pSB1C3 and pSB3K3 into Top 10 cells

Done By: Sheila and Louise
Date: 11/10 2010
Protocol: TR1.1
Notes:
Results:
Conclusion:

Retinal

Miniprep of K343005+DT in PSB1AK3

Date: 30/09
Done by: Marie
Methods: Miniprep
Protocols: MP1.1[1]
Notes:
marie skriver dette...

Restriction digest on K343002 and PSB1C3

Date: 12/10
Done by: Tommy
Methods: Restriction digest
Protocols: RD1.3[2]
Notes:
EcoRI and SpeI were used, the producted was purifided on a gel
nanodrop:
PSB1C3: 41,6
K343002: 64,5

Ligation of K343002 into PSB1C3

Date: 12/10
Done by: Marie & Christian
Methods: Ligation
Protocols: LG1.1[3]
Notes:

Transformations of K343002 in PSB1C3 into top10 E. Coli

Date: 12/10
Done by: Tommy
Methods: Ligation
Protocols: LG1.1[4]
Notes:
16 colonies and RFP control was selected from the colonie PCR. All of the colonies contain K343002 in PSB1C3
VF2 and VR primers was used
PCR Program: