ラオリ英語添削お願い

Transfer 1-1A on pSB1A10 to pSB1C3

1.estimated concentration of pSB1C3 and 1-1A which was amplified by miniprep before

2.mixed solutions according to the table below

3.incubated at 37C for 60min

4.purified the two samples

5.added 250 uL 100% EtOH to each samples

6.centrifuged at 4C, 15000rpm for 10 min

7.discarded supernatant

8.put the samples into desiccator

9.added 10 uL DW to pSB1C3 and 2 uL DW to 1-1A

10.mixed 1 uL pSB1C3 and 2 uL 1-1A

11.added 3 uL ligation solution and 0.5 uL T4 ligase

12.transfered the sample to a tube of 500 uL

13.incubated at 16C for 30 min

->For not putting 3M CH3COONa into the solutions,we stopped operation up to this.

Preparation of 3 Piece Ligation

• Parts Information

Heat Sensor

BBa_K098995 3-1E 937bp

RBS

BBa_B0034 1-2M 12bp

RFP

BBa_E1010 1-18F 681bp

Double Terminator