Team:Harvard/flavor/parts
From 2010.igem.org
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parts and primers
Primers
Name | Description | Sequence |
J45004_F | HindIII-pENTCUP2 forward primer for Expression Series insert | |
J45004_R | pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert | |
J45017_F | HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert | |
J45017_R | gusA-NheI reverse primer for Reporter Series 1 insert | |
NOSterm_BB_F | HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert | |
NOSterm_BB_R | smGFP-NheI reverse primer for Reporter Series 1 insert | |
Stop_NOSterm_BB_F | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
pENTCUP2_BB_F | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
pENTCUP2_BB_R | 1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter |