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Protocol

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Contents 1 Bacterial Work 1.1 Transformation 1.2 O/N Culture 1.3 Preparation of Competent Cells 1.4 LB Media 1.5 Preparing Plates 2 DNA 2.1 Restriction Enzyme Digests 2.2 Ligations 2.3 Mini Prep 2.4 Materials 2.5 Equipment 2.6 Procedure 2.7 Notes 2.8 References 3 Bacterial Transformation 3.1 Materials 3.2 Equipment 3.3 Procedure 3.4 Notes 3.5 References

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

DNA

Restriction Enzyme Digests

Ligations

Mini Prep

Materials

Equipment

Procedure

Notes

References

Bacterial Transformation

Materials

Equipment

Procedure

1. bah
2. bah
3. bah
4. 1bah

Notes

References