User contributions

From 2010.igem.org

• 17:34, 23 June 2010 (diff | hist) N Prepping DNA from the kit plates ‎ (New page: #With a pipette tip, punch a hole through the foil cover into the corresponding well to the Biobrick™-standard part that you want. Make sure you have properly oriented the plate. We reco...) (top)
• 17:34, 23 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→DNA)
• 17:34, 23 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→Prepping DNA from the kit plates)
• 17:34, 23 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→Prepping DNA from the Plates)
• 17:34, 23 June 2010 (diff | hist) Team:Northwestern/Protocol ‎
• 17:32, 23 June 2010 (diff | hist) N Quikchange (from primers to colonies!) ‎ (New page: ==Primers== I use [http://mekentosj.com/enzymex/ enzyme x] to work with DNA sequences, it is installed on all the macs and has nice translation and reverse complement functions. Many of ...)
• 17:29, 23 June 2010 (diff | hist) Team:Northwestern/Protocol ‎
• 17:28, 23 June 2010 (diff | hist) Team:Northwestern/Protocol ‎
• 23:37, 16 June 2010 (diff | hist) Team:Northwestern/Team ‎ (→Who we are)
• 23:34, 16 June 2010 (diff | hist) Team:Northwestern/Team ‎ (→Who we are)
• 23:29, 16 June 2010 (diff | hist) Mini Prep ‎
• 23:08, 16 June 2010 (diff | hist) Team:Northwestern/Protocol ‎
• 23:07, 16 June 2010 (diff | hist) Transformation ‎ (top)
• 17:35, 15 June 2010 (diff | hist) N Mini Prep ‎ (New page: ==Materials== For purifying plasmid DNA from ''Escherichia coli'' cells, the [http://www1.qiagen.com/Products/Plasmid/QIAprepMiniprepSystem/QIAprepSpinMiniprepKit.aspx Qiagen Spin Minipre...)
• 17:35, 15 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→DNA)
• 17:34, 15 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→DNA)
• 16:09, 15 June 2010 (diff | hist) Team:Northwestern/Team ‎ (→Help)
• 16:09, 15 June 2010 (diff | hist) Team:Northwestern/Team ‎ (→Help)
• 16:07, 15 June 2010 (diff | hist) Team:Northwestern/Team ‎
• 17:39, 14 June 2010 (diff | hist) m Transformation ‎
• 17:36, 14 June 2010 (diff | hist) O/N Culture ‎
• 17:36, 14 June 2010 (diff | hist) N O/N Culture ‎ (New page: '''REMEMBER TO BE STERILE''' *Take 5mL LB and put in a round bottom tube *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube '''MAKE SURE YOU ADD THE RIGHT ...)
• 17:33, 14 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→O/N Culture)
• 17:32, 14 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→O/N Culture)
• 17:32, 14 June 2010 (diff | hist) Preparing Plates ‎ (→Materials) (top)
• 17:31, 14 June 2010 (diff | hist) Preparing Plates ‎
• 17:30, 14 June 2010 (diff | hist) LB Media ‎
• 17:29, 14 June 2010 (diff | hist) Transformation ‎
• 17:29, 14 June 2010 (diff | hist) LB Media ‎
• 17:29, 14 June 2010 (diff | hist) Preparing Plates ‎
• 17:27, 14 June 2010 (diff | hist) Preparing Plates ‎ (→Pouring Plates)
• 17:27, 14 June 2010 (diff | hist) N Preparing Plates ‎ (New page: ==Notes== *(Optional) pre-heat a H2O bath to 55 deg C. *If you don't know how to use the autoclave, ask for a demo. ==Materials== For 1 L of Media: *10 g of Agar (for 1% Agar plates...)
• 17:23, 14 June 2010 (diff | hist) Transformation ‎ (→Transformation)
• 17:22, 14 June 2010 (diff | hist) Transformation ‎
• 17:21, 14 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→Bacterial Work)
• 17:21, 14 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→Bacterial Work)
• 16:20, 13 June 2010 (diff | hist) LB Media ‎
• 16:18, 13 June 2010 (diff | hist) LB Media ‎
• 16:18, 13 June 2010 (diff | hist) N LB Media ‎ (New page: to 950mL of deionized water add: Tryptone 10g Yeast Extract 5g NaCl 10g AUTOCLAVE)
• 16:17, 13 June 2010 (diff | hist) Team:Northwestern/Protocol ‎
• 22:38, 12 June 2010 (diff | hist) Transformation ‎
• 20:57, 12 June 2010 (diff | hist) Team:Northwestern/Notebook ‎ (→Notebook)
• 17:51, 3 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→Bacterial Work)
• 17:51, 3 June 2010 (diff | hist) Team:Northwestern/Protocol ‎
• 17:50, 3 June 2010 (diff | hist) Team:Northwestern/Protocol ‎ (→In Vitro Cloning)
• 17:50, 3 June 2010 (diff | hist) Transformation ‎
• 17:48, 3 June 2010 (diff | hist) Transformation ‎
• 17:48, 3 June 2010 (diff | hist) N Transformation ‎ (New page: #Thaw cells on ice. #Add DNA, pipette gently to mix (1μl of prepped plasmid is more than enough). #*Note: If you are adding small volumes (~1μl), be careful to mix the culture well. ...)
• 17:43, 3 June 2010 (diff | hist) m Team:Northwestern/Protocol ‎ (→Bacterial Work)
• 17:43, 3 June 2010 (diff | hist) Team:Northwestern/Protocol ‎