Team:EPF Lausanne/Notebook/week10

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Contents

1 Week 10
2 13-09-2010
3 14-09-2010
- 3.1 Cloning
- 3.2 Other
4 15-09-2010
- 4.1 Cloning
- 4.2 Other
5 16-09-2010
- 5.1 Cloning
- 5.2 Other
6 17-09-2010
- 6.1 Cloning
- 6.2 Other

Week 10

13-09-2010

Cloning

Liquid cultures for a new western started (planned for tomorrow, with new antibodies and another machine). Preparing a positive control for the western blot (bacteria expressing a his-tag-protein).

Other

Competence of E.Coli DB3.1

Movie

Continued working on movie.

14-09-2010

Cloning

Western Blot started. Samples: Immunotoxin, p25, p28, positive and negative control. Did the blot at the Lemaitre lab with Onya. Blot done twice: once with the Maerkl lab antibodies and once with Onya's antibodies.

Other

15-09-2010

Cloning

Western Blot finished. The blot with Onya's antibodies did not show any signal except a very weak band in the positive control. --> Check the size!!

Other

16-09-2010

Cloning

Other

17-09-2010

Cloning

Other

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