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Contents 1 Wetlab 1.1 Repressor group 1.2 Terminator group 1.2.1 Work flow 1.2.2 References 2 Anti-Terminator experimental setup 2.1 N-protein plasmid 2.2 References

Wetlab

On this page is described the experiences, procedures and protocols that we have used. Further the experimental process is described for the two parallel tracks, the repressor and terminator part, what have we experienced when working with our parts, and the BB standard.

Repressor group

Terminator group

[ here we should write a short abstract ]

Work flow

References

Anti-Terminator experimental setup

N-protein plasmid

The N protein were isolated from salmonella genomic DNA with specific designed primers. We used the natural occurring RBS site, as a High expression of N have shown non specific anti-termination effect on a global scale on the genome. #References References

References

• NC Franklin, JH Doelling - Am Soc Microbiol "Overexpression of N antitermination proteins of bacteriophages lambda, 21, and P22: loss of N protein specificity." - Journal of bacteriology, 1989