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From 2010.igem.org

YX's part

Picked three clones of each resistance gene and shook in 3ml LB media.

Extracted the plasmid and did PCR to verify the sequence.

The positive clones were sent for sequencing.

Module I, group 2c

Digest plasmid PSB1C13 with XbaI and SpeI, AP is used to dephosphorylate the 5' end of digestion result. digestion system

PSB1C13        15ul

10X M buffer 2ul

XbaI           1.5ul
SpeI           1.5ul

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Incubate at 37°C overnight.