Team:TU Delft/8 September 2010 content

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Revision as of 11:47, 8 September 2010 by Ptmvanboheemen (Talk | contribs)

Yay! Colonies on our Top10 plates! We're running a colony PCR as we speak to check if they contain the desired insert... stay tuned

Emulsifier

Our new construct with AlnA should be shipped by now by MrGene. Meanwhile we have optimized the protocol of the emulsification assay. In addition to the assay done last week, I now repeated the assay using only M9 and LB. This was done to see whether the medium itself already shows some background in the assay.

The experiment was done in triplicate.

TU Delft emulsification assay M9 LB.GIF

Since the background was already 0.223, opposed to about 0.12 last time, the emulsification capacity of M9 seems to be in the 0.5 mM SDS range. LB shows a higher emulsification capacity. So we decided to use K12 as the host for our emulsification production.

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