From 2010.igem.org
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PCR program: Touch-Down
| 95°C
| 1 min
|
| 95°C
| 30 sec
|
| 62°C
| 30 sec
|
| 72°C
| 30 sec
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| return to step 2 for 2 cycles
|
| 95°C
| 30 sec
|
| 60°C
| 30 sec
|
| 72°C
| 30 sec
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| return to step 6 for 2 cycles
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| 95°C
| 30 sec
|
| 58°C
| 30 sec
|
| 72°C
| 30 sec
|
| return to step 10 for 2 cycles
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| 95°C
| 30 sec
|
| 56°C
| 30 sec
|
| 72°C
| 30 sec
|
| return to step 14 for 2 cycles
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| 95°C
| 30 sec
|
| 54°C
| 30 sec
|
| 72°C
| 30 sec
|
| return to step 18 for 2 cycles
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| 95°C
| 30 sec
|
| 52°C
| 30 sec
|
| 72°C
| 30 sec
|
| return to step 22 for 2 cycles
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| 95°C
| 30 sec
|
| 50°C
| 30 sec
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| 72°C
| 30 sec
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| return to step 26 for 2 cycles
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| 95°C
| 30 sec
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| 55°C
| 30 sec
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| 72°C
| 30 sec
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| ***************
| ***************
|
| 72°C
| 10 min
|
| 12°C
| forever
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