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From 2010.igem.org

Contents 1 Nina 1.1 Inoculation of IgG protease 2 Mimmi 2.1 LMWP 2.1.1 Re-doing the PCR 2.1.2 Gels

Nina

Inoculation of IgG protease

The dish had 5 colonies, I inoculated these in 12 ml LB with 24 ul chloramphenocol resistance (50mg/ml). When I know which colony is suitable I will miniprep it and send in to iGEM hq.

Mimmi

LMWP

Re-doing the PCR

• 25 colonies were checked
  • Nina and Johan were running the PCR, I made the gels...

Gels

well	sample
1	ladder
2	1control
3	3 LMWP C
4	4 LMWP C
5	5 LMWP C (A)
6	6 LMWP C (B)
7	7 LMWP C (C)
8
9	9 LMWP N (C)

well	sample	well	sample
1	ladder	12	ladder
2	10 LMWP N (B)	13	empty
3	11 LMWP N (A)	14	18 LMWP N
4	12 LMWP N	15	empty
5	13 LMWP N	16	empty
6	14 LMWP N	17	19 LMWP N
7	15 LMWP N	18	20 LMWP N
8	16 LMWP N	19	21 LMWP N
9	17 LMWP N	20	22 LMWP N
10	empty	21	23 LMWP N
11	25 LMWP N	22	24 LMWP N