Team:Stockholm/25 July 2010

From 2010.igem.org


Contents

Nina

Inoculation of IgG protease

The dish had 5 colonies, I inoculated these in 12 ml LB with 24 ul chloramphenocol resistance (50mg/ml). When I know which colony is suitable I will miniprep it and send in to iGEM hq.



Mimmi

LMWP

Re-doing the PCR

  • 25 colonies were checked
    • Nina and Johan were running the PCR, I made the gels...


Gels

2010-07-25 pSB1C3.LMWP clone 1-9.jpg
well sample
1 ladder
2 1control
3 3 LMWP C
4 4 LMWP C
5 5 LMWP C (A)
6 6 LMWP C (B)
7 7 LMWP C (C)
8
9 9 LMWP N (C)


2010-07-25 pSB1C3.LMWP clone 10-25.jpg
well sample well sample
1 ladder 12 ladder
2 10 LMWP N (B) 13 empty
3 11 LMWP N (A) 14 18 LMWP N
4 12 LMWP N 15 empty
5 13 LMWP N 16 empty
6 14 LMWP N 17 19 LMWP N
7 15 LMWP N 18 20 LMWP N
8 16 LMWP N 19 21 LMWP N
9 17 LMWP N 20 22 LMWP N
10 empty 21 23 LMWP N
11 25 LMWP N 22 24 LMWP N





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/