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Contents 1 pSB1C3 plasmid gel electrophoresis 1.1 Aims 1.2 Materials and Protocol 1.3 Result 1.4 Discussion 1.5 Conclusion

pSB1C3 plasmid gel electrophoresis

Aims

The aim of this experiment is to run gel electrophoresis for the extracted and linearized plasmid pSB1C3 fragment which were amplified at 4 different melting temperatures by 4 separate PCR reactions.

Materials and Protocol

Please refer to: gel electrophoresis for gel electrophoresis protocol.

Result

Figure 1: Gel electrophoresis of the amplified linearized plasmid pSB1C3 fragments ran at 4 different melting temperatures, Tms, (50, 60, 65, 70°C). A 1 kb DNA ladder was used on either side of lanes.

• Lane 1: pSB1C3 fragment amplified at 55°C
• Lane 2: pSB1C3 fragment amplified at 60°C
• Lane 3: pSB1C3 fragment amplified at 65°C
• Lane 4: pSB1C3 fragment amplified at 70°C
• Lane 5: pSB1C3 fragment amplified at 55°C
• Lane 6: pSB1C3 fragment amplified at 60°C
• Lane 7: pSB1C3 fragment amplified at 65°C
• Lane 8: pSB1C3 fragment amplified at 70°C

Discussion

Conclusion