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Team:Uppsala-SwedenWeek7
From 2010.igem.org
Week-7
Preparation for getting the bricks:
(1)Competent cell preparation
(2)Transformation of the Biobricks into DH5-alpha competent cells
(3)Verificiation of the lengths of the biobricks by Gel Electrophoresis after PCR.
Details of the biobricks we are using:
|
Biobricks details |
||||||||||||
|
Part BBa_ |
Name |
Length (bp) |
Plasmid Length |
Total Length |
Plasmid |
Plasmid number |
Plate |
Well |
Antibiotics |
Sequencing |
Quality (Gel, Q, P) |
Function |
|
F 2621 |
F2 |
1158 |
2079 |
3237 |
PSB1A2 |
All |
2 |
21F |
Amp |
Confirmed |
Bad, OK, OK |
Sensor |
|
F 1610 |
F1 |
798 |
3189 |
3987 |
pSB1Ak3 |
AHL Relay |
2 |
24G |
Amp & Km |
Confirmed |
OK, OK, OK |
AHL generator |
|
I 746350 |
F17 |
237 |
2079 |
2316 |
pSB1A2 |
1 & 4 |
2 |
12C |
Amp |
Partially |
OK, OK, OK |
Activator in sensitivity tuner |
|
I 746352 |
F4 |
264 |
2079 |
2343 |
pSB1A2 |
2,3,5 & 6 |
2 |
12G |
Amp |
Partially |
OK, OK, OK |
Activator in sensitivity tuner |
|
B0015 |
F5 |
129 |
3189 |
3318 |
pSB1AK3 |
All |
1 |
23L |
Amp & Km |
Confirmed |
OK, OK, OK |
Terminator |
|
I746360 |
F6 |
91 |
2079 |
2170 |
pSB1A2 |
1 & 4 |
2 |
12I |
Amp |
Confirmed |
OK, OK, OK |
Lower level promotor |
|
I746364 |
F7 |
93 |
2079 |
2172 |
pSB1A2 |
2 & 5 |
2 |
12O |
Amp |
Confirmed |
OK, OK, OK |
Lower level promotor |
|
I746365 |
F8 |
92 |
2079 |
2171 |
pSB1A2 |
3 & 6 |
2 |
14A |
Amp |
Inconsistent |
OK, OK, OK |
Lower level promotor |
|
Q04510 |
F9 |
987 |
4425 |
5412 |
pSB2K3 |
All |
1 |
18B |
Km |
Confirmed |
OK, Low, OK |
Inverter |
|
P0412 |
F10 |
1308 |
2079 |
3387 |
pSB1A2 |
All |
3 |
6P |
Amp |
Confirmed |
OK, OK, OK |
Lower level repressor |
|
I746361 |
F11 |
92 |
2079 |
2171 |
pSB1A2 |
1,2,4 & 5 |
2 |
12K |
Amp |
Confirmed |
OK, OK, OK |
Higher level promotor |
|
R0010 |
F12 |
200 |
2079 |
2279 |
pSB1A2 |
4,5 & 6 |
1 |
1D |
Amp |
Confirmed |
OK, OK, OK |
Regulatory promotor |
|
I0460 |
F16 |
969 |
3189 |
4158 |
pSB1AK3 |
4,5 & 6 |
2 |
24I |
Amp & Km |
Partially |
OK, OK, OK |
AiiA generator |
|
I13504 |
F13 |
875 |
2079 |
2954 |
pSB1A2 |
1 (GFP) |
1 |
22I |
Amp |
Confirmed |
OK, High, OK |
GFP Generator |
|
I13507 |
F14 |
861 |
2079 |
2940 |
pSB1A2 |
2 (RFP) |
1 |
22O |
Amp |
Confirmed |
OK, High, OK |
RFP Generator |
|
E0430 |
F15 |
878 |
2079 |
2957 |
pSB1A2 |
3 (YFP) |
1 |
8I |
Amp |
Confirmed |
OK, High, OK |
YFP Generator |
|
B0034 |
F3 |
12 |
2079 |
2091 |
pSB1A2 |
4,5 & 6 |
1 |
2M |
Amp |
Confirmed |
OK, High, OK |
RBS |
Gel image of the biobricks after confirmation of lengths:
