Team:Newcastle/15 July 2010
From 2010.igem.org
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Screening for lacI insert:
- (Miniprep)
- Restriction digests - the restriction digest protocol was followed.
- Single digest with Pst1
- double digests with Pst1 and Xba1
- Gel electrophoresis
Set up liquid broth culture in 5 ml of LB