Team:Newcastle/23 June 2010

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Contents 1 Wednesday Morning 1.1 Aims 1.2 Equipment List 1.3 Transformation 2 Wednesday afternoon taking a break 2.1 Aims 2.2 Materials

Wednesday Morning

Aims

The aim of today's Lab practice was to transform competent cells with GFP and RFP inserts in an RFP backbone.

Equipment List

• E.coli broth culture
• Pipettes
• Plasmid ligation products from yesterday
• Conical flasks

Transformation

Cells are made competent ready for transformation.

• Positive control(plasmid uncut)- to make sure cells are transfroming properly
• Negative control (just cells)- nothing but competent cells

5 microfuge tubes:

• 1:5
• 1:3
• Vector
• Negative add nothing
• Positive add 1μl uncut

Wednesday afternoon taking a break

Aims

The aim of this Lab practice was to have fun.

Materials

• Candy floss
• Suntan lotion
• Teddy bears
• Burgers and bacon
• Chips and gravy
• Icecream
• Meerkat toys
• Madmouse ride!
• Beer, wine, lemonade
• Football

	Call to arms!	Philamentous!!!