Bioluminescence

The Plan

• Characterise the transfer function of PoPs + CHBT --> Light

• The Luciferin Cycle:

• [http://www.ncbi.nlm.nih.gov/pubmed From paper on isolation of H-LRE and G-LRE]

[Previous attempts to improve light production of luciferase reaction - Pyruvate orthophosphate dikinase (PPDK) converts AMP + PPi to ATP (AMP + PPi + Phosphoenol Pyruvate -> ATP + Pyruvate + Phosphate) => light emission intensified in presence of low ATP concentration]

Sequences

• [http://www.ncbi.nlm.nih.gov/nucleotide/14331151?report=genbank&log$=nucltop&blast_rank=1&RID=4EUAXMJW01R A-LRE (P.pyralis) Sequence]
• [http://www.ncbi.nlm.nih.gov/nuccore/AB261988.1 Mutant Luciferase (P.pyralis) Sequence]