Week of 9/27
From 2010.igem.org
September 29th
- Colony PCR:
-LuxR+J23100 -LuxR+J23108 -LuxR+J23116
An extension time of 1 hour and 5 minutes was used.
September 30th
The oxygen sensing promoters were run today!
Gel order:
-ladder -D1 -D2 -D3 -D4 -D5 -J6 -J7 -J8 -J9 -J10
October 2nd
After experiencing some minor problems, we re-ran the oxygen sensitive promoters. This time around we had great success!!
- Gel Order:
-Ladder -D1 -D2 -D3 -D4 -D5 -J6 -J7 -J8 -J9 -J10