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From 2010.igem.org

September 29th

• Colony PCR:

-LuxR+J23100
-LuxR+J23108
-LuxR+J23116

An extension time of 1 hour and 5 minutes was used.

September 30th

The oxygen sensing promoters were run today!

Gel order:

-ladder
-D1
-D2
-D3
-D4
-D5
-J6
-J7
-J8
-J9
-J10

October 2nd

After experiencing some minor problems, we re-ran the oxygen sensitive promoters. This time around we had great success!!

• Gel Order:

-Ladder
-D1
-D2
-D3
-D4
-D5
-J6
-J7
-J8
-J9
-J10

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