Team:WITS-South Africa/parts

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Contents 1 Mission Statement 2 List of Parts Submitted to The Registry 2.1 Non-Composite Parts 2.1.1 Machine 1 Parts 2.1.2 Machine 2 Parts

Mission Statement

Here at WITS, we’re serious about our synthetic biology; we like knowing that when you shove your (nitrile-gloved) hand into our box of bio bits you emerge with a fully-functional nut for your bolt. So, hence, therefore, as a result we have decided to provide the entire life cycle of our BioBricks - over and above their behavioral characterization. From their genesis to gene synthesis, we’re giving you it all: snapshots of their sequences to our many guesses at their gels. Finally, you can rest assured that they sit happy in their bubble-wrapped plasmid packaging as we submit them off into the great lake of science - The Registry.

List of Parts Submitted to The Registry

Non-Composite Parts

Machine 1 Parts

• Lac1/AraC Promoter: The inducible promoter serving as a proxy for infection
• PlcR-PapR Fusion Protein: The positive-feedback signal-propagating quorum molecule
• Venus: The YFP variant used in fluorometric analysis

Machine 2 Parts

• PlcR Promoter: The PlcR-PapR fusion protein-responsive promoter
• SpooA: The negative feedback regulator of the machine

At Wits, we love our Biobricks!