Team:METU Turkey/Results Discussion/Isothermal Titration Calorimetry (ITC)

From 2010.igem.org

CLSM IMAGING

- Take 10mL from the samples (OD=1-1.9) that are dissolved in phosphate buffer solution.
- Spread the samples on the slide with the help of an inoculation loop.
- Wait for the sample to dry.
- Pass the slide through the flame 3-4 times, being careful to have the cells on the upper part of the slide.
- Observe the slides under Brightfield 100X

-Settings for GFP:

                            
HFT 488 
                            
Ch 1
                            
LP 505 
                            
Line 8
                            
543 nm excitation
                            
Laser power 5%

- Settings for RFP:

                            
HFT 488/543 
                            
Ch 1
                            
LP 560 
                            
Line 8
                            
543 nm excitation
                            
Laser power 10%