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Team:Warsaw/Stage3
From 2010.igem.org
BactoDHL
Our goal was to create an intracellular protein delivery platform, as efficient as accurate. Such tool could have the whole variety of applications ranging from scientific research to prevention or therapy of disease. In our attempt to achieve that goal, Escherichia coli K12 laboratory strain was equipped with molecular machinery enabling successful mammalian cell entry. This is how BactoDHL was born.
The bacterium attaches to the outer membrane of a mammalian cell by means of invasin also responsible for ingestion into a forming endosome. There, aided by lysteryolisyn it escapes entrapment and rests in the cytosol.
We strongly encourage you to get familiar with the background of BactoDHL and steps of it's design. Please visit results and gallery of microphotographs to find out how it behaves in living cells.
