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Team:Newcastle/Meetings/26 March 2010
From 2010.igem.org
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| Minutes for formal meeting 26th March 2010 |
| * Room: CBCB; Start: 11 am |
| * Chair: Jannetta, Minutes: Rachel, Computer: Harsh |
| 1. Roll call and apologies |
| Jen, Janetta, Neil, Steven,Younus, Da, Alan, Richard, Harsh |
| Phil – Holiday |
| Apologies - Jem and Matt |
| 2. Approval of agenda and minutes |
| Approved |
| 3. Brief summary of previous meeting |
| Tutorial |
| 4. Research Feedback |
| 1. Properties of concrete and surface tension (Zoltan and Steven)
High compressive strength low tensile strength Steal added good for temp change Caco3 – similar to concrete; expand same as concrete... might crack like concrete Spider silk like steal- reduce possibility of cracking Glue- no many papers- expansion may not be a problem? Silk alkali resistant Capillary properties of concrete change over time- in project we consider small rapid Cement water ratio and surface area and environment Where to look for the cracks? Crack pattern |
| 2. Microinjection (Zoltan and Steven) |
| Simple issue
Not correct term Swimming Bacillus Oxygen gradient big selling points |
| 3. Genetics Homologous recombination plasmid chromosome (Harsh and Rachel) |
| Test in plasmids- Find good plasmid
Integrate into genome Recombination site... Find!!! amyE site amylase with insert can’t break down amylase |
| 4. Nutrients (Harsh and Rachel) |
| Bacteria algae growth living in concrete
Only activate feed bacillus Two hybrid Bio films exclude other not algae Antifungals, anti algals, Antibiotic |
| 5. Sporulation, germination and secretion control (Alan and Da) |
| SpoA – last years team sporulation control, maybe turn off completely
Nutrient availability KinA Inducible promoter |
| Glue out of bug? |
| 6. Quorum sensing (Younus and Phil) |
| Compx
And cff Two component system comp |
| Why ? Don’t want?- not die after job fill new cracks
Nuclease Phil masEF Skf SbB If linear DNA works |
| 8. Glue, CaCo3 and spider silk (Zoltan and Jannetta)
Bacteria that produce CaCo3 from urea How secrete spider silk long complex proteins – how can we make it happen in bacillus Secretion systems |
| 9. Other points |
| Linear DNA – Richard
Justifications of Linear Build it up the use. Mutations- How stop general questions Not specifically a problem – keep thinking about it though |
| 5. Action points |
| All -Read Voygt paper |
| All-Next week working off the proper wiki!!! |
| Janetta contact details doc in drop box |
| Janetta and Richard – Flow chart!!! |
| Reminder to neil and jen not welcome trust funding by 2nd of April? |
| Janetta- T-shirt sponsors and logo – embroidered / print mixed – 5 or 6 sponsors design your own- printing cheaper , quotes dependent on design |
| Janetta- send us links for graphic design software- please |
| Transfer the wiki |
| 1. Individual iGEM applications |
| Phil hasn’t applied |
| Richard not accepted |
| Janetta text Phil |
| Jen/Neil-Pay for team!! |
| 2. Visas/Passport update |
| Harsh- May June apply |
| Richard visa waiver |
| 3. Wiki transfer |
| 1. Clarify contents of identified pages |
| 2. Id Ethics page |
| Research page |
| Safety page |
| entify other pages required |
| 3. Ideas for layout / theme |
| Zoltan Tidy it up-Urgent |
| Borrow last years / Look at anything thats nice |
| It evolves!!! We can change it!!! Now!!!!!!! |
| 4. Travel plans |
| 1. Flights |
| Need to sort out who is going |
| 2. Accomodation |
| Need to sort out who is going |
| 5. Funding |
| Harsh has made draft letter |
| Main chemical company – free stuff ? |
| 6. Advisors/additional team members - civil engineer/graphics designer? |
| Still looking –Neil,Jen |
| 7. Tutorials |
| Sequentia and Clone manager- basics |
| Modelling part two |
| 8. What next? |
| Flow chart of what we’re going to do Big picture!!! |
| If its coloured life is easier |
| Cambridge brick-Colour |
| Web services for Biosynthetics, Flow chart |
| Deverner |
| 9. Time line discussion |
| Need someone else on time line. |
| 16th of June Lab induction |
| Deadlines soon |
| Change flight deadline – not 31st |
| 6. Other business |
| Pdf mark up tools – pdf exchange viewer, foxit, keeps mark up |
| Making dropbox use better |
| 7. Items for next agenda |
| Need wiki transferred by next meeting!!! |
| Review of flow chart if done |
8. Next meeting- Thursday early as possible – email organise
1. Chair-Da
2. Minutes-Alan
3. Computer-Younus
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