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From 2010.igem.org

Wet lab

• Part Design • Lab Work • Beads • Safety • Future Work •

Our main achievement in the wet lab has been the creation of a new composite biobrick, engineering E. coli to produce GFP in response to Nitrates. Not only is this part complete, but it's also well characterised both on its own and working in tandem with a second biobrick.

We also developed a way of encapsulating our bacteria, allowing not only an easy and safe method of spreading them in the environment, but also dramatically improving signal detection and E. coli survival in soil.

Finally we worked to further characterise the PyeaR promoter submitted by Edinburgh in 2009, improving the quality of information available in the parts registry.

In this section you can find out about the design of our parts, including all the decisions we made and why, as well as all the information relating to their construction and characterisation. It also contains material on our new encapsulation methods, and finally lists all our safety considerations both in the lab and on a wider environmental scale.

For a more in depth view of what we have done so far, click on the Notebook section.

For a complete list of the biobricks used during our project, plus a brief description on their use, click here.

Subpage Structure

Team:BCCS-Bristol/Wetlab/Part_Design

Team:BCCS-Bristol/Wetlab/Beads

Team:BCCS-Bristol/Wetlab/Improvements

Team:BCCS-Bristol/Wetlab/Experiments

Team:BCCS-Bristol/Wetlab/Safety

Team:BCCS-Bristol/Wetlab/Project_Results

Team:BCCS-Bristol/Wetlab/Image_Gallery

Links to old pages

Team:BCCS-Bristol/Wetlab/Notebook

Team:BCCS-Bristol/Wetlab/BioBricks

Team:BCCS-Bristol/Wetlab/Lab_photos