Team:WITS-South Africa/Machine Design

Lacto-detect

Purpose:

a) To act as the ‘Detector’ Machine within the population and produce the quorum signalling peptide in response to an input signal

So how did we end up selecting the final parts of our Machines? Each one was chosen after much consideration and scouring of the literature to select the most suitable biological system. For a design rationale of why we selected the parts that we did to create this machine, click here

For an account of how we assemblied and verified Lacto-detect, click here

Lacto-report

For a design rationale of why we selected the parts that we did to create this machine, click here

We were not able to finish constructing Lacto-report in it's entirety, due to time constraints and issues with the E.chromi Biobrick parts. (For more details, click here.)

For testing and characterising the behaviour of the quorum sensing mechanism in a model Gram-positive bacillus, we used an intermediate machine construct we dubbed Lacto-

Intermediate Machines

Intermediate Machine 2 (Figure 1) Purpose: a) To characterise the strength of the induction of the Lac/Ara-1 promoter by measuring fluorescence after IPTG is added b) To show that the PlcR promoter is activated in L. gasseri by measuring fluorescence after the addition of exogenous PlcR and PapR proteins

Machine Testing