Firstly we want to thank and acknowledge our sponsors below. This project would not be possible without you!

Tsinghua University


School of Life Science in Tsinghua University


Department of Physics, School of Sciences, Tsinghua University


Academy of Arts and Design, Tsinghua University


Office of International Cooperation and Exchange
Office for Affairs of Hong Kong,Macao and Taiwan
Tsinghua University


Tsinghua University Academic Affair Office


Center of Theoretical Biology

Stem Cell Research Center in Tsinghua University

We thank José Torres Díaz¹ and Julio González² for their efforts to help us translate our Project Background into Spanish.

We thank WANG Shiqi³ for Japanese translation amending and LIANG Zecai³ for support, ZHANG Ying⁴ for Russian translation, QI Weijie⁵ for Japanese translation, Monika⁶ for German translation.

1. From School Electrical & Electronic Engineering, University of Tarapacá. Arica, Chile.

2. From School of Industrial Engineering, University of Tarapacá. Arica, Chile.

3. From School of Life Science, Tsinghua University

4. From Dalian University of Foreign Languages, now in РГСУ, Российский Государственный Социальный Университет.

5. From Shanghai International Studies University

6. From Denmark and got her Bachelor Degree in Germany.

1. Stephan Frey, Martin Haslbeck, Otmar Hainzl and Johannes Buchner: Synthesis and characterization of a functional intact IgG in a prokaryotic cell-free expression system Biol. Chem., Vol. 389, pp. 37–45

2. Thomas E. Kuhlman ,Edward C. Cox:Site-specific chromosomal integration of largesynthetic constructs. Nucleic Acids Research, 2009, 1–10.

3. Alex Pautsch and Georg E. Schulz Structure of the outer membrane protein A transmembrane domain Nature Structural biology 1998,5,11, 1013-1017

4. JOSEPH A. FRANCISCO* Transport and anchoring of 8-lactamase to the external surface of Escherichia coli PNAS 1992 89, 2713-2717

5. Keren Ofir, Yevgeny Berdichevsky Versatile protein microarray based on carbohydrate-binding modules Proteomics 2005, 5, 1806–1814

6. Ming-xiong He, Hong Feng, Yi-zheng Zhang, Construction of a novel cell-surface display system for heterologous gene expression in Escherichia coli by using an outer membrane protein of Zymomonas mobilis as anchor motif, Biotechnol Lett (2008) 30:2111–2117

7. Yariv Mazor, Thomas Van Blarcom, Robert Mabry, Brent L Iverson & George Georgiou, Isolation of engineered, fulllength antibodies from libraries expressed in Escherichia coli, NATURE BIOTECHNOLOGY

8. Yariv Mazor, Thomas Van Blarcom, Brent L Iverson & George Georgiou, E-clonal antibodies: selection of full-length IgG antibodies using bacterial periplasmic display, NATURE PROTOCOLS 1766 VOL.3

9. Christian W. Cobaugh, Juan C. Almagro, Mark Pogson, Brent Iverson and George Georgiou, Synthetic Antibody Libraries Focused Towards Peptide Ligands, J. Mol. Biol. (2008) 378, 622–633

10. Micka¨el Desvaux, The cellulosome of Clostridium cellulolyticum Enzyme and Microbial, Technology 37 (2005) 373–385

11. SANDRINE PAGE`S ANNE BE´LAI¨CH , HENRI-PIERRE FIEROBE, CHANTAL TARDIF, CHRISTIAN GAUDIN,AND JEAN-PIERRE BE´LAI¨CH, Sequence Analysis of Scaffolding Protein CipC and ORFXp, a New Cohesin-Containing Protein in Clostridium cellulolyticum: Comparison of Various Cohesin Domains and Subcellular Localization of ORFXp, JOURNAL OF BACTERIOLOGY, Mar. 1999, p. 1801–1810

12. Sachdev S Sidhu, Full-length antibodies on display, NATURE BIOTECHNOLOGY VOLUME 25 NUMBER 5 MAY 2007

13. Sang Yup Lee, Hyun Uk Kim, Jin Hwan Park, Jong Myung Park and Tae Yong Kim, Metabolic engineering of microorganisms: general strategies and drug production, Drug Discovery Today _ Volume 14, Numbers 1/2

14. Ario de Marco, Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli, Microbial Cell Factories 2009, 8:26