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From 2010.igem.org

Lab Work

We tried to compensate for yesterdays delays. We transformed 15 Biobricks from the distribution plates!

Some PCR reactions were performed on short BioBrick inserts which would eventually save time in the long-run (compared to transforming and plasmid isolation). The BioBricks involved were J61100, J61101, J61107, J61117, J61127 and B0034.

BB template (10x diluted)

The PCR reaction was run at varying annealing temperatures to figure out which temperature gave the best result. After analysis on gel we deduced that a temperature of 50 degrees Celsius was optimal.

At the end of the day we performed a Restriction digestion reaction on the amplified PCR products as well as the previously isolated I13401 and pSB3C5.

Mixtures were incubated at 37 degrees for 2.5 hours and stored at 4 degrees overnight.