Team:Caltech/Protocols

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  • Making V1012 competent
  • Making stock solutions
  • Freezer (glycerol) stocks
  • Plating antibiotics
  • Sequencing primers
  • Making synthase casette
    • Sources of sequences
    • Optimizing & checking genes
  • Chemical/Crosslinking reactions
    • Digesting substrate (hydrolysis) + epoxidation (MCPBA)
    • crosslinking (AIBN3) + UV initiation
  • Inducing lysis via 632nm light initiation
  • Making cell/glycerol/agar mixture
    • Applying this to plates
  • Growing cells with PHA (and substrate oil)
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