Team:Heidelberg/Notebook/miMeasure/August

08/08/2010

seeding cells for test measurements - 96 well plate for plate-reader and FACS

test of media:

• OptiMEM 0% FBS + Pen/Strep + L-Glu + other according to cell line
• OptiMEM 2% FBS + Pen/Strep + L-Glu + other according to cell line
• DMEM 10% FBS+ Pen/Strep + L-Glu + other according to cell line

• cells did not adhere properly so we decided to coat 96-well plate with poly-L-lysine and repeat
• significantly better growth in DMEM with 10% FBS, so we decided to grow cells in it and before measurement to change it to OptiMEM, to reduce high background fluorescence coming from FBS.