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Protocol/3
From 2010.igem.org
Protocol 3: Glycerol Stock
What you will need:
- Overnight with bacterial growth (probably 1.5 mL)
- Screw cap tubes
- Glycerol
Procedure:
- Pipet 150 uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
- Add 350 uL of overnight culture to each tube.
- Pipet up and down to gently mix.
- Flash freeze in liquid N2 or dry ice/methanol bath
- Place in -80 C freezer when frozen.
Notes
* If we make extra we have them if we need to send them out or want to store in several locations.
