BacillaFilla: Fixing cracks in Concrete

Project Description

BacillaFilla, an engineered Bacillus subtilis, aims to repair cracks in concrete, which can cause catastrophic structural failure. BacillaFilla would be applied to structures by spraying onto their surface.

BacillaFilla swims deep into the cracks. Repair is effected by production of CaCO₃, filamentous Bacillus subtilis cells and levan. CaCO₃ expands at the same rate as concrete, making it the ideal filler. Filamentous Bacillus subtilis cells have similar tensile strength to the synthetic fibres used in fibre-reinforced concrete, and provide reinforcement. Levan glues CaCO₃ and filamentous cells in place.

B. subtilis 168 sporulates, making it ideal for storage and transportation. The cells are naturally tolerant to concrete's high pH.

We designed a swarming BioBrick part for repairing B. subtilis 168's defective swrA and sfp genes and regaining motility. At the end of the crack the quorum sensing peptide subtilin triggers a co-ordinated population response from a [Team:Newcastle/End_of_crack_%26_signalling_system#2008Brick|subtilin-inducible promoter]. Upregulating SR1 and rocF promotes arginine and urea production, increasing exogenous CaCO₃ deposition. Over-producing YneA induces the filamentous cell phenotype, while SacB converts extracellular sucrose to levan glue.

To protect the environment our project will also include a design for a kill switch.

Summary of achievements:
Bronze:	From the beginning of the project, we have successfully registered the team of two instructors, six advisors and eight members. We have completed and submitted a Project Summary form, came up with ideas and shared them on our iGEM wikipedia page. We also entered information on one new BioBrick part, the yneA BioBrick into the Registry of Parts. The BioBrick for the IPTG-induced filamentous cell formation has been submitted to the iGEM Registry.
Silver:	Our new BioBrick, the IPTG-inducible filamentous cell formation part works, so we characterised it and included the information, http://partsregistry.org/Part:BBa_K302012:Experience BBa_K302012, on the Parts Registry.
Gold:	In order to obtain a gold, we investigated the benefits of an e-Science approach, focusing on workflows, to synthetic biology. Details can be found here.