Team:Brown/Protocols

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Protocols

Here are the protocols that we followed. Occasionally we make exceptions to the general "recipe," but those exceptions are documented in our Notebook. PCR

PCR

Ligations

Ligation

Restriction digests

Restriction Digest

Overnights

Overnight Culture

Transformations

Bacterial Cell Transformation

Cell competence protocols

Media:Protocol_Quick_CaCl2_competence.pdf