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From 2010.igem.org

Home The Team The Project Parts Submitted to the Registry Modeling Notebook Safety Collaboration

Contents 1 Notebook 1.1 6/16/10 1.2 6/21/10 1.3 6/23/10 1.4 6/24/10 1.5 6/25/10 1.6 6/25/10

Notebook

6/16/10

Prepared nine pots of wildtype (Col-0) A. thaliana and moved to cold storage for stratification. Pots intended for use in Agrobacterium flower-dip. Seeds were sewn in soilless media and the pot was covered with a wide meshing to retain the soil.

6/21/10

Transfered pots from cold storage to greenhouse. Prepared 0.5 MS agar media and sterilized seeds for sterile growth, to be sewn on 6/23. Seeds left in dilute agar solution in fridge to stratify.

6/23/10

Agar plates poured. Seeds to be sewn on 6/24 to give time for the media to solidify.

6/24/10

Seeds sown onto agar. Five large culture plates and two small plates were then transfered into horticulture growth chamber. Seeds in soil media forecasted to germinate by 6/26.

6/25/10

A. thaliana expirement mapping: Testing low oxygen response in WT and Transgenic using atmospheric chamber in Purdue Agronomy department. Using sensors to measure ethanol, oxygen, auxin response in control and in hypoxic conditions.

6/25/10

Communicating with IU iGEM team concerning synergies including arabidopsis transformation workshop.

Communicating with Naperville, Ill high school student as part of community outreach experience.

Planning Purdue University campus poster session to involve public in forum discussion on genetic engineering.