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AUGUST, 27TH
PLATE
EXPERIMENT DESCRIPTION
Motivation
This experiment was performed in LB to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. (see Protocol) of the promoters from Anderson's collection in high copy number plasmids.
Methods
Accordign to Protocol 2 the experiment was performed in LB on the following parts:
- <partinfo>BBa_J23100</partinfo>
- <partinfo>BBa_J23101</partinfo>
- <partinfo>BBa_J23105</partinfo>
- <partinfo>BBa_J23106</partinfo>
- <partinfo>BBa_J23110</partinfo>
- <partinfo>BBa_J23114</partinfo>
- <partinfo>BBa_J23116</partinfo>
- <partinfo>BBa_J23118</partinfo>
- <partinfo>BBa_B0031</partinfo> (negative control)
RESULTS
It is possible to split the promoters into 3 groups: strong promoters (<partinfo>BBa_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters(<partinfo>BBa_J23118</partinfo> and <partinfo>BBa_J23110</partinfo>) and weak promoters (the other ones).
| Promoter | R.P.U. (average) | std
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| <partinfo>BBa_J23100</partinfo> | 0.989 | 0.025
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| <partinfo>BBa_J23101</partinfo> | 1 | -
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| <partinfo>BBa_J23105</partinfo> | 0.071 | 0.004
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| <partinfo>BBa_J23106</partinfo> | 0.122 | 0.008
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| <partinfo>BBa_J23110</partinfo> | 0.583 | 0.016
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| <partinfo>BBa_J23114</partinfo> | 0.037 | 0.006
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| <partinfo>BBa_J23116</partinfo> | 0.043 | 0.009
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| <partinfo>BBa_J23118</partinfo> | 0.341 | 0.009
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