Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test27agosto

From 2010.igem.org

AUGUST, 27TH



PLATE


UNIPV Pavia piastra27agosto2.png


EXPERIMENT DESCRIPTION

Motivation

This experiment was performed in LB to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. (see Protocol) of the promoters from Anderson's collection in high copy number plasmids.

Methods

Accordign to Protocol 2 the experiment was performed in LB on the following parts:

RESULTS


UNIPV Pavia risHCLB.png


It is possible to split the promoters into 3 groups: strong promoters (BBa_J23100 and BBa_J23101), medium strength promoters(BBa_J23118 and BBa_J23110) and weak promoters (the other ones). It is notable that BBa_J23110 resulted stronger than BBa_J23118.

Promoter R.P.U. (average) std
BBa_J23100 0.989 0.025
BBa_J23101 1 -
BBa_J23105 0.071 0.004
BBa_J23106 0.122 0.008
BBa_J23110 0.583 0.016
BBa_J23114 0.037 0.006
BBa_J23116 0.043 0.009
BBa_J23118 0.341 0.009



Tecan Test