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Protocol

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OpenWetWare Protocols: http://openwetware.org/wiki/Protocols

Contents 1 DNA 1.1 Prepping DNA from the Plates 1.2 Quikchange (from primers to colonies!) 2 Bacterial Work 2.1 Transformation 2.2 O/N Culture 2.3 Preparation of Competent Cells 2.4 LB Media 2.5 Preparing Plates 3 DNA 3.1 Restriction Enzyme Digests 3.2 Ligations 3.3 Mini Prep

DNA

Prepping DNA from the Plates

Quikchange (from primers to colonies!)

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

DNA

Restriction Enzyme Digests

Ligations

Mini Prep