Team:Northwestern/Notebook

Brainstorming April-June 2010

Week1 6/13/10-6/19/10

Week2 6/20/10-6/26/10

Week3 6/27/10-7/3/10

Week4 7/4/10-7/10/10

Week5 7/11/10-7/17/10

Week6 7/18/10-7/24/10

Week7 7/25/10-7/31/10

Week8 8/1/10-8/7/10

Week9 8/8/10-8/14/10

Week10 8/15/10-8/21/10

Week11 8/22/10-8/28/10

Week12 8/29/10-9/4/10

Week13 9/5/10-9/11/10

Week14 9/12/10-9/18/10

Week15 9/19/10-9/25/10

Week16 9/26/10-10/2/10

Week17 10/3/10-10/9/10

Week18 10/10/10-10/16/10

Week19 10/17/10-10/23/10

Week20 10/24/10-10/30/10

10/3/10

Timi miniprepped the CP-LacPI-GFP cultures and digested them with E/P. She then poured a big and a small gel.

Kevin came in and ran the digested CP-LacPI-GFP, digested CP-LacPI with digested Cp and digested LacPI parts.

10/4/10

Timi and Kevin decided to take a day off :)

10/5/10

Timi and Kevin came in to discuss why their gels were running strangely. We believe there is something wrong with our buffer, so we are swapping everything out with a fresh batch of Prof. Mordacq's 0.5x TBE buffer. Timi poured a large gel that we will be using tomorrow.

10/6/10

10/7/10

Timi- took part 2-17F out of the DNA kit. Transformed into T10 cells to grow overnight.

10/8/10

Kevin- ran gel of the CP-LacPI parts. Gel results are still inconclusive. Also put in an overnight culture of the 2-17F part into the incubator for Timi.

10/9/10

Timi- Miniprepped the 2-17F part. Digested the DNA with E/P and ligated the 2-17F GFP part into Chlor and Tet backbone.

10/10/10

Timi- Transformed and plated part 2-17F onto Chlor and Tet plates. Will be ready for miniprepping tomorrow.

10/11/10

10/12/10

Matt - digested LacP-RBS with SpeI in preparation for standard assembly with ES digested CHS3

10/13/10

10/14/10

Kevin transformed T10 cells with CP-LacPI parts that we did not have much digest of left. He plated them onto TET plates.

10/15/10

Kevin took out the CP-LacPI plates and put them into the 4 degree for Timi. Timi inoculated overnight cultures at 7pm.

10/16/10

Timi and Kevin miniprepped the CP-LacPI inoculations, digested them with E&P, and ligated them to GFP.

10/18/10

Matt miniprepped 31GFP, 21GFP (old), and 32GFP (old). He digested the miniprepped DNA with E/P for Kevin to run on a gel. He also reinoculated all 9 CP-LacPI combinations.