Team:Harvard/flavor/parts
From 2010.igem.org
parts and primers
Primers
Name | Description | <
J45004_F | Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Forward primer |
J45004_R | Wintergreen pathway part. Encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase I. Reverse primer |
J45017_F | Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Forward primer |
J45017_R | Wintergreen pathway part. Encodes an isochorismate pyruvate-lyase and an isochorismate synthase. Reverse primer |
NOSterm_BB_F | Plant specific terminator forward primer |
NOSterm_BB_R | Plant specific terminator reverse primer |
Stop_NOSterm_BB_F | NOSt plant specific terminator with stop codon on end |
pENTCUP2_BB_F | Plant specific promoter forward primer |
pENTCUP2_BB_R | Plant specific promoter reverse primer |
Parts
Name | Description |
pENTCUP2 promoter | Plant specific promoter. Used to drive expression of our flavor constructs in plants. |
NOSt terminator | Plant specific terminator. Terminates transcription but no stop codon on end. |
NOSt terminator + stop | Plant specific terminator. Terminates transcription with stop codon on end. |
Brazzein | Sweet-tasting protein. 54 amino acid sequence. |
Miraculin | Taste-inverting protein turns sour to sweet. 191 amino acid sequence |
Brazzein YFP2x C-terminus | Brazzein with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression |
Miraculin YFP2x C-terminus | Miraculin with two Yellow Fluorescent Protein sequences on C-Terminus. Allows visualization of expression |
Brazzein StrepII C-terminus | Brazzein with StrepII tag on C-terminus for Western Blot. |
Miraculin StrepII C-terminus | Miraculin with StrepII tag on C-terminus for Western Blot. |